This paper deals a novel method for the synthesis of silver nanoparticles (AgNPs) by microwave assisted approach using silver nitrate and trisodium citrate. The formation of nanoparticles was confirmed by UV-visible spectral studies. Particle size and morphology was determined by P-XRD and HR-TEM studies which revealed the average particle size of synthesized nanoparticles 21.49 and 20.08 nm respectively with spherical shape. The synthesized nanoparticles were screened for antibacterial activity in vitro against gram-positive bacteria Staphylococcus aureus and Bacillus subtilis and gram-negative bacteria Escherichia coli and Klebsiella pneumoniae by adopting disk diffusion method. The results of antibacterial studies exhibited that AgNPs were potential antibacterial agent.

Keywords: Nanoparticles; Microwave Irradiation; Antibacterial Activity;