This paper addressing the synthesis (hydrothermal route) and comparative study of TiO₂ nanocrystals from two different sources TTIP (titanium tetra isopropoxide) and TiO₂ powder. TiO₂ nano crystals having excellent properties and can be used in various applications including glass window self-cleaning, purification of air, water and antimicrobial coating by tapping the photo catalytic properties of nano particles. The synthesized titania was characterized by SEM, XRD, FTIR and UV-Vis spectroscopy. The particles size calculation was done by applying Scherrer equation. The resulted size of titania particles was vary from 20-100 micrometers. The crystal size of particles are vary from 10-47 nanometers.