The stock solution of reactive blue 49 (RB 49) made up 1000 ppm in mile pure water. The voltammetric behaviour of RB 49 has been studied in aqueous solution on glassy carbon and modified glassy carbon electrode. The influence of pH on the cyclic voltammetric behaviour of Reactive Blue 49 was studied from 1.0 to 13.0 at scan rate 50 mV/s. The maximum peak current response was found in pH 7.0. The cyclic voltammogram of RB 49 was reveled one anodic peak and one cathodic peak at potential around 731 mV and –846 mV vs Ag/AgCl. A systematic study of the experimental parameters that affect the anodic stripping voltammetric response was carried out. Maximum peak current conditions were arrived. Calibration curve was made under the maximum peak current conditions. The concentration range studied for the determination of 250 to 700 ppb. The lower limit of detection is 200 ppb for GC and 100 ppb for Graphine and the RSD 2.2% and 1.8% respectively. The suitability of this method for the quantisation of dye in textile industries effluents was also ascertained.

Keywords: Cyclic Voltammetry; Reactive Blue 49; Stripping Voltammetry; Glassy Carbon Electrode; Graphene;