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Article – Journal of Nanoscience and Technology

Journal of Nanoscience and Technology, Volume 3,Issue 1,2017 Pages 245-248

Synthesis, Characterization and Cytocompatibility of HfO2 Nanoparticle Against A549 Cell Lines with Potent Antimicrobial Activities
M. Jayavel*, N. Ramalakshmi, S. Arul Antony

The rapid development of nanotechnology, the nanomaterials start to cause people’s attention for potential toxic effect. In this paper the hafnium oxide nanoparticles (HfO2 NPs) have been successfully synthesized by precipitation method using the precursors of HfCl4 and NaOH. The synthesized materials were characterized by using X-ray diffraction (XRD), UV–visible and Transmission Electron Microscope (TEM). In addition with the cytocompatibility of Hafnium oxide nanoparticles against A549 (Human Lung Cancer Cell) cell lines was investigated. Also the synthesized compound was tested against Gram-positive and Gram-negative bacterial strains. The XRD analysis revealed the presence of monoclinic HfO2 nanoparticles. The absorption peak at 212 nm can be assigned to absorption of HfO2 nanoparticles. The TEM image showed that the HfO2 NPs were of spherical in shape with particle size of about 10 nm. The Mitochondrial Toxicity Test (MTT) assay method was employed to assess cell viability after exposure to 10, 25, 50, 100 and 250 μg/mL of HfO2 NPs for 24 h. Cell viability does not affect when the particles size at 10 nm of HfO2 NPs and to the exposure level at 10 μg/mL. The disc diffusion method was adopted to test the antibacterial activity of HfO2 nanoparticles exhibit better antibacterial activity for the Gram negative bacterial K. pneumoniae and the Gram positive bacterial Staphylococcus aureus.

Keywords: Hafnium Oxide; MTT Assay; A549 Cell Line; Nanoparticles; Precipitation Method;