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Article – Journal of Natural Products and Resources

Journal of Natural Products and Resources, Volume 2,Issue 2,2016 Pages 65-67

Isolation of Steroid from Unsaponifiable Portion of Trichilia emetica Seed Oil
A. Usman*, V. Thoss, N. Liman Usman, M. Haruna

Trichilia emetica (Vahl) seed oil has a wide folk medicinal usage in Africa. The aim of this study was to phytochemically investigate the unsaponifiable portion of Trichilia emetica seed oil. The portion was subjected to repeated column chromatography and preparative thin layer chromatography (PTLC) using hexane, chloroform, ethyl acetate and methanol. Final purification was achieved using an isocratic gradient elution of CHCl3 and CH3CN at a ratio of 3 to 1, in Reveleris Flash System fitted with C18 column. The structure of the isolated compound was elucidated on the basis of high resolution mass spectrometry as well as 1D and 2D NMR spectroscopic data analysis. Also, by direct comparison of the data obtained with those reported in the literature. It was then resolved that the compound isolated was stigmasterol, a steroid present in plant fats or oils. This compound has not been previously reported from this species.

Keywords: Trichilia emetica; Phytochemistry; Unsaponifiable; Isolation; Steroid;