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Article – Journal of Natural Products and Resources

Journal of Natural Products and Resources, Volume 2,Issue 2,2016 Pages 76-78

Mechanism of Action of Acaciasides on Microsomes of Setaria cervi
S. Datta*, S.P. Sinha Babu

Cell membranes are made up of unsaturated lipids which are susceptible to oxidative damage. Oxidative damage can lead to a breakdown or even hardening (peroxidation) of lipids. It is firmly established that homogenates of most animal tissues form thiobarbituric acid reactants (peroxides) when incubated in vitro in aerobic conditions. These substances are formed by the oxidation of unsaturated fatty acids of lipid constituents of the tissue probably via hydroperoxides. Acaciaside A and B are triterpeniod saponins originally isolated from the funicles of Acacia auriculiformis. Acaciasides A and B contain a conjugated unsaturated system which is involved in the the damaging effects of saponins probably by resulting free radicals that labilize parasite membrane through peroxidation. Saponins have significantly enhanced membrane peroxidation in terms of both MDA and CD formation. The increased rate of peroxidation may lead to the formation of peroxyl radicals that may react with the lipid, probably by hydrogen abstraction. To further investigate the mechanism of saponins-induced membrane damage, SOD, thiourea and catalase were added to microsome membranes of Setaria cervi prior to the addition of saponins. It is evident that superoxide anions are probably involved in the expression of membrane damaging effect of saponins.

Keywords: Lipid Peroxidation; Acaciasides; Membrane Damage; Setaria cervi; Superoxide Anion;