This study investigated the total phenolic and the different antioxidant vitamins contents in the ethanol and acetone extracts of the leaf of Acalypha wilkesiana using standard analytical methods, in order to authenticate the medicinal potentials of this plant. The results obtained indicated that ethanol extracted the highest phenolic content. The analysis of vitamin concentration showed that acetone extract had the highest vitamins A and E contents (0.812±0.45 and 108.97 ± 0.79 mg/100 g respectively), while the ethanol extract contained the highest concentration of vitamin C (79.595±0.926 mg/100 g). Results from this study have shown that Acalypha wilkesiana leaves are a good source of antioxidant - vitamins and polyphenols, and are potential source of natural antioxidants.