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Article – Journal of Natural Products and Resources

Journal of Natural Products and Resources, Volume 1,Issue 1,2015 Pages 13-16

In Vitro Antioxidant and Hepatoprotective Effect of Leaf Galls of Terminalia chebula Retz., Against CCl4 Induced Oxidative Stress in Liver Slice Culture Model
A.A. Rajopadhye, A.S. Upadhye*

The galls on Terminalia chebula Retz. (Family - Combretaceae) is a well-known herbal drug used to treat various diseases in Indian System of Traditional Medicine. The dried galls are widely commerce in the Indian market and substituted to galls of Pistacia integerrima Stew. Ex. Brandis. The present study aims to investigate antioxidant and hepatoprotective activity of hexane (TCH), ethanol (TCE) and water extract (TCW) of the galls on T. chebula by employing photochemiluminescence and spectrophotometric methods. The results showed that TCE and TCW significantly inhibited 2,2-diphenyl-1-picrylhydrazyl, nitric oxide and superoxide radical in dose dependent manner. Trent of phenol content was as: TCH < TCW < TCE. A significant correlation was shown by total phenol content and free radical scavenging activity of all extracts. The hepatoprotective effect was assayed in CCl4-induced cytotoxicity in a liver slice culture model. The results revealed that significant depletion was observed in lactate dehydrogenase, lipid peroxidation, antioxidative enzymes superoxide dismutase, catalase, and Glutathione reductase on administration of the TCE and TCW or ascorbic acid as standard in the CCl4-induced cytotoxicity in the liver. TCE and TCW extracts of galls on T. chebula have prevented significant oxidative liver damage.

Keywords: Terminalia chebula Retz.; Oxidative Stress; Liver Slice Culture Model;