Phytochemical screening of the extracts has shown the presence of steroids, flavonoids, saponins, glycosides, tannins, phenolic compounds, fixed oils, and fats in Jurinea dolomiaea root extracts. The presence of lupeol has been reported previously by us using high-performance thin-layer chromatography and high-performance liquid chromatography. Present research studies encompasses identification of chemical constituents in Jurinea dolomiaea roots of methanol extracts by hyphenated technique such as gas chromatography-mass spectroscopy (MS) which when coupled gives a clear insight of constituents. The components were identified by matching mass spectra with MS libraries. There were 5 different compounds analyzed from Jurinea dolomiaea roots. The identified components are (2,4-ditert-butylphenyl)-5-hydroxypentanoate, 2‑ethylhexylheptadecyl sulphite, 6-methyltridecane, (9E, 12E)-9,12-Octadecadienoyl chloride, Linoleic acid chloride, linoleoyl chloride, Lup-20 (29)-en-3-ol Lup‑20(29)‑en‑3β‑ol Lup‑20(29)‑en‑3α‑ol Lupeol, (3α)‑isomer Lupeol, (3β,18β,19β)‑isomer, in Jurinea dolomiaea root extracts.