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Article – Journal of Pharmaceutical and Medicinal Research

Journal of Pharmaceutical and Medicinal Research, Volume 2,Issue 2,2016 Pages 55-59

Effects of Ferulic Acid in Rotenone Induced Rat Model of Parkinson’s Disease
C. Liu*, R. Wang, T. Ji, Y. Fan, Z. Qin, X. Gao

Parkinson’s disease (PD) is an age-related neurodegenerative disorders. In order to explore novel agents for treatment of PD, we have evaluated the neuroprotective efficacy of Ferulic Acid (FA) using rotenone-induced rat model of PD. Rotenone was administered 2.5 mg/kg b.wt to male Wistar rats for four weeks to induce the PD. The paradigm for evaluating FA was based on chronic administration for four weeks at the dose of 50 mg/kg, 30 min prior to rotenone administration. In our study, rotenone administration caused significant reduction in endogenous antioxidant like superoxide dismutase, catalase and glutathione. Rotenone challenge induced lipid peroxidation evidenced by increased malondialdehyde (MDA) following perturbation of antioxidant defense. Apart from oxidative stress, rotenone also activated proinflammatory cytokines and enhanced inflammatory mediators such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). The immunofluorescence analysis revealed a significant increase in the number of activated microglia and astrocytes accompanied by significant loss of dopamine neurons (DA) in the substantia nigra pars compacta (SNc) area upon rotenone injection. However, treatment with FA rescued DA neurons in SNc area and nerve terminals in the striatum from the rotenone insult. FA treatment also restored antioxidant enzymes, prevented depletion of glutathione and inhibited lipid peroxidation. Following treatment with FA, the inflammatory mediators such as COX-2 and iNOS and proinflammatory cytokines were also reduced. Further, the results were supported by a remarkable reduction of Iba-1 and GFAP hyperactivity clearly suggests attenuation of microglial and astrocytic activation. These results suggests that FA has promising neuroprotective effect against degenerative changes in PD and the protective effects are mediated through its antioxidant and anti-inflammatory properties.

Keywords: Parkinson’s Disease; Rotenone; Neurodegeneration; Neurotoxicity; Ferulic Acid;

Journal of Pharmaceutical and
Medicinal Research