Nanomedicine is widely explored nowadays for treatment of life threatening diseases, yet comes with various challenges and questions. The present study encapsulates the acute toxicological aspects of the Euphorbia thymifolia coupled silver nanoparticles (ET-Ag NP). For acute toxicity studies according to OCED (Organization for Economic Cooperation Development) guidelines Swiss Albino mice (6-8 weeks) were used and were given single intraperitoneal dose of 2000 and 5000 mg/kg body weight and were observed for mortality and other side effects for 14 days. The individual components of the formulation, viz. silver oxide in surface modified nano form and at low dose, and Euphorbia thymifolia are both biocompatible materials. No changes were found for general appearance, behavior and body weight, thus concluding that the nanocomposite formulation does not have single dose toxicity.