In this study, simple approach was applied for the synthesis, characterization and application of silver nanoparticles from the leaves of Bryophyllum p., garlic and jamun which is cost-effective, eco-friendly and easy to synthesis. The extract acts both as reducing as well as capping agent. The color change of the solution yellowish brown to reddish brown confirms the formation of silver nanoparticles. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy which showed a peak between 420 and 430 nm corresponding to the absorbance of the AgNPs that confirmed the reduction of Ag⁺ to Ag⁰ nanoparticles. The anti-bacterial activity of AgNPs was investigated against Xanthomonas axonopodis pv. punicae and its identity was confirmed by morphological, biochemical and pathogenicity test. The results indicated that garlic AgNPs shoed maximum inhibition at a concentration of 15 mM. Hence, they can be used in the disease management in pomegranate.

Keywords: Pomegranate; Bacterial Blight Disease; Xanthomonas axonopodis; Silver Nanoparticles;