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Article – Journal of Advanced Electrochemistry

Journal of Advanced Electrochemistry, Volume 2,Issue 2,2016 Pages 70-74

A Novel Hybridization Indicator Zn2(ET)(pzdc)(μ3-OH) for the Electrochemical Determination of Breast Cancer Gene BRCA1
L. Liu, F. Sun, Y. Wang, C. Ding, H. Li*

A novel and facile biosensor using Zn2(ET)(pzdc)(μ3-OH) complex as hybridization indicator was developed and further applied to detect breast cancer DNA. The (Nb, Mn) codoped titanium oxide (TMN) and carboxylated multi-wall carbon nano-tube (MWCNT-COOH) was immobilized on the electrode surface to prepare the composite film modified electrode, fixed single-stranded DNA (ssDNA) on the modified electrode surface with EDC and NHS as activator. Then a novel and sensitive electrochemical DNA biosensor based on the TMN and MWCNT-COOH composite film was prepared. The properties of the modified electrode and the interaction between indicator and DNA were studied by scanning electron microscopy (SEM), fluorescence spectrum, UV-vis spectrum, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, the target single-stranded DNA (ssDNA) could be quantified ranging from 1 × 10-14 M to 1 × 10-6 M with good linearity (r = 0.9991) and a low detection limit of 1.67 × 10-15 M(3σ, n = 11). Results showed that the synergistic effect between TMN and MWCNT-COOH could improve the sensitivity of the biosensor, which has great potential application for fabrication of electrochemical biosensor.

Keywords: Electrochemical DNA Biosensor; Zn2(ET)(pzdc)(μ3-OH); MWCNT-COOH; Breast Cancer Gene;