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Article – Journal of Advanced Electrochemistry

Journal of Advanced Electrochemistry, Volume 2,Issue 2,2016 Pages 78-84

A Green Approach to the Synthesis of Eriochrome Black-T Capped Silver Nanoparticles and Its Electrochemical Detection of L-Tryptophan and L-Tyrosine in Blood Sample and Antibacterial Activity
E. Prabakaran, V. Sheela Violet Rani, A. Brabakaran, K. Pandian, D. Jesudurai*

A new development and green approach was adopted for the synthesis of Eriochrome Black-T stabilized silver nanoparticles (AgNPs@EBT) in the presence of sunlight is described. The resulting AgNPs@EBT was isolated and then characterized with Fourier Transform Infrared Spectroscopy (FT-IR), UV-Visible spectroscopy (UV-Vis), Powder X-ray Diffraction Spectroscopy (PXRD) and Field Emission Scanning Electron Microscopy (FE-SEM). The electrochemical behavior of the EBT stabilized silver nanoparticles modified glassy carbon electrode (AgNPs@EBT/GCE) was investigated using a redox probe of K3Fe(CN)6. The electro-catalytic characteristics of AgNPs@EBT/GCE were used for the electrochemical oxidation of L-Tryptophan (L-Trp) and L-Tyrosine (L-Tyr) in the presence of 0.1 M PBS as supporting electrolyte. The AgNPs@EBT/GCE exhibits high electrocatalytic activity towards the oxidation of both L-Trp and L-Tyr. The AgNPs@EBT/GCE shows a linear response to L-Trp between 0.1 × 10-6 M and 10 × 10-5 M and for L-Tyr, it is between 0.1 × 10-6 M and 0.9 × 10-6 M. The detection limit for L-Trp and L-Tyr is 9.84 × 10-8 M and 9.81 × 10-8 M, respectively. In addition, the modified AgNPs@EBT/GCE displays good sensor activity and making it suitable for the determination of L-Trp and L-Tyr in blood samples. The antibacterial activity of AgNPs@EBT synthesized by the green method was tested against gram negative and gram positive micro-organisms. There is an enhanced antibacterial activity was observed with AgNPs@EBT, when compared with EBT alone.

Keywords: AgNPs@EBT; Electrocatalytic Oxidation; L-Tryptophan; L-Tyrosine; Antibacterial Activity;