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Article – Journal of Advanced Electrochemistry

Journal of Advanced Electrochemistry, Volume 1,Issue 1,2015 Pages 19-24

Electrochemical Study of Some Schiff Base by Cyclic Voltammetry and Its Metal Complex - DNA Interaction Study by UV-Visible Spectroscopy
R. Das*, A. Saxena, S. Saxena, G. Khan

Present paper investigate the electrochemical behaviour of isatin-3-hydrazone derivative i.e. A, B, C mentioned in manuscript and its interaction with salmon sperm fish DNA. Electrochemical study of these compounds done in 0.02 M phosphate buffer and 0.1 M LiCl used as a supporting electrolyte at carbon pest electrode by cyclic voltamtery (CV). CV study reveal that all synthesized compound show 2e- diffusion control, irreversible reduction peak and the product of this reduction are secondary amines. The diffusion coefficients of these derivatives were also calculated. After that DNA-interaction study of its metal (Cd) complex done by UV-Visible spectrophotometer that show metal cation (Cd+2) or protonated nitrogen atom of these metal complexes probably interact with O atom of phosphate group in DNA back bone by electrostatic attraction force and the result of these type of interaction are denaturation of DNA strand which seem by hyperchromism in the emission spectra of these complex. Binding/association constants of these complexes were also calculated in this paper.

Keywords: Schiff Base; Carbon Paste Electrode; Cyclic Voltamtery; DNA interaction; UV-Visible Spectroscopy;